Leicester Riders and Sevenoaks Suns claimed the two Semi-Final spots from the South, while Westfield Health Sheffield Hatters maintained their perfect record in the competition.
Sevenoaks started the day with a convincing 51-97 triumph away at Oaklands Wolves with four double-doubles from the visitors including 27 points and 12 rebounds from Renee Busch.
The Riders followed suit and claimed top spot with a resounding 46-77 success on the road against the previously unbeaten Barking Abbey Crusaders, who miss out in the three-way tie.
Sheffield improved to 4-0 in the North as they held off a late rally from Team Northumbria to win 84-94 behind 25 points apiece from Helen Naylor and Steph Gandy.
WBBL Trophy Semi-Finals
Hatters v Suns
Riders v Wildcats